Distribution and depletion of [14C]resmethrin isomers administered orally to laying hens

The cis and trans isomers of the synthetic pyrethroid resmethrin, labelled with radiocarbon in either the alcohol or acid moiety, were individually administered orally to White Leghorn laying hens at a dosage of 10 mg kg^?1. With each isomer and label position, greater than 90% of the radiocarbon was eliminated in the excreta within 24 h after the treatment. Radiocarbon residues in the egg white and yolk fractions were low, with peak levels observed 1 and 4-5 days after treatment in white and yolk, respectively. In birds sacrificed 12 h after treatment, radiocarbon residues in tissues were low; the highest levels were found in the liver and kidney.     

抗生素对火炬松的器官发生植株再生的影响(英文)

本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验的3种抗生素提高愈伤组织的形成和芽再生,但降低了芽的生根频率。这些结果表明,选择合适的抗生素对优化火炬松遗传转化体系有重要作用。图3表4参25。     

Plant biotechnology: A case study ofBacillus thuringiensis (Bt) and its application to the future of genetic engineered trees

Agricultural productivity may be raised in a sustainable way by many different technologies such as biological fertilizers, soil and water conservation, biodiversity conservation, improved pest control, and changes in land ownership and distribution. Of these measures, biotechnology applications probably hold the most promise in augmenting conventional agricultural productivity, because biotechnology applications give not only the need to increase production, but also protect the environment and conserving natural resources for future generations. Biotechnology applications will have the possibilities to increase productivity and food availability through better agronomic performance of new varieties, including resistance to pests; rapid multiplication of disease-free plants; ability to obtain natural plant products using tissue culture; diagnosis of diseases of plants and livestock; manipulation of reproduction methods increasing the efficiency of breeding; and the provision of incentives for greater participation by the private sector through investments. Insect resistance through the transfer of a gene for resistance fromBacillus thuringiensis (Bt) is one of the most advanced biotechnology applications already being commercialized in many parts of the world. This paper reviews the development and the status ofBt technology and application ofBt transgenic plants in current agriculture, and discusses specific issues related to the transfer of the technology to the future of genetic engineered trees with emphasis on conifers. Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, Department of Biology, Howell Science Complex, East Carelina University, Greenville, NC 27858-4353, USA. Responsible editor: Chal Ruihai     

与生长激素相关的高等植物细胞周期生化调控

高等植物细胞周期是细胞生物学上的重要研究领域。细胞周期受遗传和发育影响因素的调控。本文综述高等植物细胞周期中涉及到细胞周期关卡GO-G1-S期和G2-M期的激素调控。最新研究成果证明玉米素、腺嘌呤、激动素、赤霉素和脱落酸等通过细胞周期蛋白依赖性蛋白激酶及细胞周期关卡的脱磷酸化控制细胞周期。植物D-型细胞周期蛋白也已被证明在细胞周期调控中起关键作用。本文有关细胞周期的研究综述对植物细胞和发育生物学研究有参考价值。图3参61。     

Differential expression of genes encoding cell wall proteins in vascular tissues from vertical and bent loblolly pine trees

Differential expression of cell wall proteins during plant development and in response to biotic or abiotic stress suggests that these proteins may contribute in different ways to plant cell wall architecture. Because the wood of loblolly pine (Pinus taeda L.) is highly specialized in the formation of secondary cell walls, it is an ideal tissue for studying these proteins. The cDNAs coding for six novel cell wall associated proteins, as well as a homologue for a phytocyanin, were identified and characterized from differentiating xylem of loblolly pine. Three of these cDNAs encoded new putative loblolly pine arabinogalactan proteins, based on their structural similarity to classical arabinogalactan proteins (AGPs). In addition, one clone was related to the proline-rich protein group and the other two to the glycine-rich protein group and the mussel adhesive protein. Relative expression of these genes was examined in different tissues and organs from normal trees (needles, phloem and vertical wood), the underside of bent trees (compression wood) and the lateral sides of the bent stems (side wood). All clones, except one, were highly expressed in vascular tissues with noticeable differences among the three types of wood. Their relationships and diversity provide the first insights into concerted expression and related function for this important group of proteins in cell wall formation of wood.     

Diurnal changes in water conduction in loblolly pine (Pinus taeda) and Virginia pine (P. virginiana) during soil dehydration

We studied diurnal changes in water conduction during soil dehydration in 37-month-old seedlings of one Virginia pine (Pinus virginiana Mill.) and two loblolly pine (P. taeda L.) sources, one from North Carolina (NC) and the other from the "Lost Pines" areas of Texas (TX), in an environmentally controlled growth chamber. For seedlings of similar biomass, the TX source had higher values of transpiration, needle conductance, and plant hydraulic conductivity under well-watered conditions than the NC source. Under dry soil conditions, the TX source had lower values of water conduction than the NC source. The Virginia pine source responded similarly to the TX source under both well-watered and dry soil conditions. For all three pine sources, gradients between soil and needle water potentials were greatest when the seedlings were moderately stressed. The TX and Virginia pine sources had higher gradients and lower daytime needle water potentials under moderate stress conditions than the NC source. Predawn needle water potentials did not differ among the pine sources. We conclude that the TX and Virginia pine sources have decreased daytime needle water potentials and increased water potential gradients during the daytime under moderate stress conditions, but with no disruption of recovery at predawn. The greater rates of transpiration and water conduction by the TX source compared with the NC source under well-watered conditions suggest a means by which growth can be maximized prior to the onset of drought, thereby enhancing survival of loblolly pines in drought-prone environments.     

The biochemical control of the cell cycle by growth regulators in higher plants

The cell cycle is an important research field in cell biology and it is genetically and developmentally regulated in animals and plants. The aim of this study was to review knowledge about the biochemical regulation of the cell cycle by plant growth regulators through molecular checkpoints that regulate the transition from G0-G1-S-phase and G2-M in higher plants.Recent research has shown that zeatin treatment led to the up-regulation of CycD3 in Arabidopsis. Benzyladenine treatment can also shorten the duration of S-phase through recruitment of latent origins of DNA replication. Kinetin is involved in the phosphoregulation of the G2-M checkpoint; the major cyclin-dependent kinase (Cdk) at this checkpoint has recently shown to be dephosphorylated as a result of cytokinin treatment, an effect that can also be mimicked by the fission yeast Cdc25 phosphatase. Gibberellic acid (GA) treatment induces internode elongation in deepwater rice, this response is mediated by a GA-induced up-regulation of a cyclin-Cdk at the G2-M checkpoint. Recent evidence has also linked abscisic acid to a cyclin-dependent kinase inhibitor. A new D-type cyclin, recently discovered in Arabidopsis may have a key role in this process. A brief review on plant growth regulator-cell cycle interfacing during development and a cytokinin-induced continuum of cell cycle activation through the up-regulation of a plant D-type cyclin at the G1 checkpoint and the phosphoregulation of the Cdk at the G2/M checkpoint had been concluded. This review could be valuable to research on cell and developmental biology in plants.     

植物生物技术:苏云金杆菌抗虫基因的研究和它对未来树木遗传工程的影响

许多不同的技术可以促进农业生产力的持续增长，生物技术是其中最重要和最有前途的方法。因为生物技术不仅可以增加生产力。而且在环境和自然资源的保护中起重要作用，应用生物技术可以生产高产、优质、高抗性和无病植物，同时也可以提高育种效率和促进食品的工业化生产。苏云金杆菌抗虫基因是目前生物技术领域中应用最广泛的例子之一。由此而产生的抗虫植物正在广泛应用于世界各地的商业化生产。本文综述了苏云金杆菌抗虫基因的研究进展和它在未来林木遗传工程中的应用。表4参68。     

The effects of temporal differences between map and ground data on map-assisted estimates of forest area and biomass

Key message

When areas of interest experience little change, remote sensing-based maps whose dates deviate from ground data can still substantially enhance precision. However, when change is substantial, deviations in dates reduce the utility of such maps for this purpose.

Context

Remote sensing-based maps are well-established as means of increasing the precision of estimates of forest inventory parameters. The general practice is to use maps whose dates correspond closely to the dates of ground data. However, as national forest inventories move to continuous inventories, deviations between map and ground data dates increase.

Aims

The aim was to assess the degree to which remote sensing-based maps can be used to increase the precision of estimates despite differences between map and ground data dates.

Methods

For study areas in the USA and Norway, maps were constructed for each of two dates, and model-assisted regression estimators were used to estimate inventory parameters using ground data whose dates differed by as much as 11 years from the map dates.

Results

For the Minnesota study area that had little change, 7-year differences in dates had little effect on the precision of estimates of proportion forest area. For the Norwegian study area that experienced considerable change, 11-year differences in dates had a detrimental effect on the precision of estimates of mean biomass per unit area.

Conclusions

The effects of differences in map and ground data dates were less important than temporal change in the study area.

     

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) progress to date and future plans

The INHAND Proposal (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) has been operational since 2005. A Global Editorial Steering Committee (GESC) manages the overall objectives of the project and the development of harmonized terminology for each organ system is the responsibility of the Organ Working Groups (OWG), drawing upon experts from North America, Europe and Japan.Great progress has been made with 9 systems published to date – Respiratory, Hepatobiliary, Urinary, Central/Peripheral Nervous Systems, Male Reproductive and Mammary, Zymbals, Clitoral and Preputial Glands in Toxicologic Pathology and the Integument and Soft Tissue and Female Reproductive System in the Journal of Toxicologic Pathology as supplements and on a web site – www.goreni.org. INHAND nomenclature guides offer diagnostic criteria and guidelines for recording lesions observed in rodent toxicity and carcinogenicity studies. The guides provide representative photo-micrographs of morphologic changes, information regarding pathogenesis, and key references. During 2012, INHAND GESC representatives attended meetings with representatives of the FDA Center for Drug Evaluation and Research (CDER), Clinical Data Interchange Standards Consortium (CDISC), and the National Cancer Institute (NCI) Enterprise Vocabulary Services (EVS) to begin incorporation of INHAND terminology as preferred terminology for SEND (Standard for Exchange of Nonclinical Data) submissions to the FDA. The interest in utilizing the INHAND nomenclature, based on input from industry and government toxicologists as well as information technology specialists, suggests that there will be wide acceptance of this nomenclature. The purpose of this publication is to provide an update on the progress of INHAND.